NATIVE: Nanobody-assisted tissue immunostaining for volumetric EM

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining

Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions. Minimizing the incubation time in fixative reduces disruption of tissue morphology, and reducing the incubation time in staining solution or antibody solution decreases nonspecific labeling. Reduction of incub...

متن کامل

A Haptic-Assisted Guidance System for Navigating Volumetric Data Sets

In this paper, a new approach for navigation assistance through sets of volumetric data is presented. This innovative method uses force feedback in two dimensions to guide the user to a region of interest. The haptic feedback is calculated under two methods and we explain the implementation of both approaches. The output is displayed by the Proactive Desk, a two dimensional force feedback devic...

متن کامل

Incentives for P2P-Assisted Content Distribution: If You Can’t Beat ’Em, Join ’Em

The rapid growth of content distribution on the Internet has brought with it proportional increases in the costs of distributing content. Adding to distribution costs is the fact that digital content is easily duplicable, and hence can be shared in an illicit peer-to-peer (P2P) manner that generates no revenue for the content provider. In this paper, we study whether the content provider can re...

متن کامل

Whole-mount Fixation and Immunostaining of Brain Tissue

Immunocytochemistry on sectioned tissue is a powerful method for determining the cellular and subcellular distributions of an epitope. However, where the expression is patterned, understanding the distribution often requires extensive, time-consuming threedimensional reconstruction with all its attendant artifacts. The techniques described in this unit circumvent these problems. The distributio...

متن کامل

Allograft Replacement for Absent Native Tissue

CONTEXT Structural instability due to poor soft tissue quality often requires augmentation. Allografts are important biological substitutes that are used for the symptomatic patient in the reconstruction of deficient ligaments, tendons, menisci, and osteochondral defects. Interest in the clinical application of allografts has arisen from the demand to obtain stable anatomy with restoration of f...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Protocol Exchange

سال: 2018

ISSN: 2043-0116

DOI: 10.1038/protex.2018.108